Start Date
24-4-2025 12:00 AM
Description
Over-the-counter pain relievers often contain multiple active ingredients that can be analyzed by High Performance Liquid Chromatography (HPLC). A method developed and optimized to separate acetaminophen, aspirin, and caffeine from these tablets. The contents of Excedrin pain relievers and the generic counterpart from Walgreens, Walmart, and CVS were tested. Optimization examined multiple mobile phases, temperatures, and run times for the procedure. The results were compared to analytically prepared standards as well as the packaging information, with the primary goal of creating a reproducible and efficient protocol for HPLC instrumentation. The method successfully separated the contents of all materials reliably within a 15 minute protocol.
Recommended Citation
Pritchett, Dylan; Lewis, Joshua; and Firestine, Jennifer, "HPLC Method Development for the Separation and Quantification of Common Pain Relievers" (2025). 2025 Student Academic Showcase. 23.
https://digitalcommons.lindenwood.edu/src_2025/Posters/Posters/23
Included in
HPLC Method Development for the Separation and Quantification of Common Pain Relievers
Over-the-counter pain relievers often contain multiple active ingredients that can be analyzed by High Performance Liquid Chromatography (HPLC). A method developed and optimized to separate acetaminophen, aspirin, and caffeine from these tablets. The contents of Excedrin pain relievers and the generic counterpart from Walgreens, Walmart, and CVS were tested. Optimization examined multiple mobile phases, temperatures, and run times for the procedure. The results were compared to analytically prepared standards as well as the packaging information, with the primary goal of creating a reproducible and efficient protocol for HPLC instrumentation. The method successfully separated the contents of all materials reliably within a 15 minute protocol.